Flow cytometry systems is an intracellular device. The flow can be analyzed up to 20 parameters. Flow cytometry is commonly used in academic studies and for the diagnosis and follow-up of diseases associated with the patient’s immune system. Sample preparation, instrument calibration, data collection and data analysis.
The flow cytometry systems consists of 4 steps.
Sample Preparation: At this stage, first of all blood is drawn from the person or from the subject (which can be animal). Mononuclear cells are separated from the blood sample of the recipient and these cells are marked with special biomarkers. Instrument Calibration: In accordance with the biomarkers to be used during the calibration phase, so many of the controller inspections and inspection samples are calibrated. It’s the power of device calibration to experiment. Data Collection: At this stage, the cell count is passed through the tube contained in the cytometer and a single cell transition is provided in front of the laser. The collision of the cell and the laser beam emits the resulting light. The scattering from the field differs according to the size, internal structure and biological properties of the cell. Data Acquisition: To obtain various scatterings with radiation values sent to the computer. These charts use bioinformatics experts to determine if the group of cells they are looking for is at a steady rate, and if so, at what rate.
Cells and cytometer
In contrast to the biochemical and physical properties of cells that have been determined for many years using primer microscope features, flow cytometry techniques have begun to be used today. Flow cytometry is a technique and device based on the principle that the various cells pass one by one through a flow channel as a suspension and are classified according to cell size and granularity with flow cytometry systems. With this device, the surface and inner proteins, organelles and other components of the cell are analyzed and distinguished by means of laser and electronic technology based on size, granularity and fluorescence emission. We do not know the basics of the flow cytometry technique. This technique will enable us to use many pathologies and increase the standardization of the results and diagnostic value.
The technique is fast, capable of counting thousands of particles at a time (allowing single-celled fluorescent features to be counted very quickly in very short time). It is possible to analyze with high sensitivity (it is possible to count each particle size separately for each wavelength). The variation constant (CV = standard deviation / mean) for light scatter and fluorescence measurements of uniform microspheres is less than 1%.
Flow cytometers typically only measure peak or integrated signals, not advanced structural details. This problem can be solved by optically active chemistries which are bound to all the constituents other than the structure which is specifically bound to the desired particles or to be examined. Many flow cytometers analyze very small volumes (<0.5 mm3). However, the cells are at least about 103 / ml. For this reason, the flow cytometry device must be calibrated and calibrated correctly in flow cytometry systems.